Synthetic Gene Synthesis

We synthesize the gene, clone and sequence verify
Good clone with no mutations delivered
Gene  expression/ protein purification services available Cat. # GEPS 01

SB/SGS 01

Full-Length cDNA Cloning

Full-length cDNA from any organism
Based on partial cDNA information, consensus sequence data or homologous organism sequence data
Sequence confirmed clone delivered
Synthetic synthesis is also available, Cat. # SGS 01
Gene expression/ protein purification services available Cat.# GEPS 01

SB/FLCS 01

Development and Screening of cDNA /EST Library/ SSH Library

cDNA library developed from all sources
1,000 positive clones selected
All 1,000 clones are sequenced
All cDNA clones with annotated sequence data provided
Sequencing of more or fewer samples

SB/CDN 01

Gene expression and Protein purification services

Genes are expressed in bacterial and Pichia and Mammalian systems based systems
Proteins are purified for antibody development, Crystallography, assay development, etc.
Proteins are solubilized and re-folded to active form as well
Milligram to Gram Scale
Protein expression and purification in bacterial expression system, ~5 mg
Protein expression and purification in Pichia expression system, ~5 mg
Protein expression and purification in Mammalian expression system
Baculovirus/ Insert cell line system

SB/GEPS 01
SB/GEPS 02

SB/GEPS 03
SB/GEPS 04

Solubilization of insoluble protein

Starting from insoluble / inclusion body, proteins are extracted and solubilized for activity
Different refolding protocols are tried and provided to Scientists for assays
More protein is solubilized following the same protocol (that performed on assay)
Biological activity not guaranteed

SB/SIP 01

Gene Engineering Services

Development of Deletion mutants, Chimeras, Knock-out constructs, Constructs with repeat elements, etc
Development of Cloning/ Expression vectors

SB/GES 01

Mutagenesis Services

Site-Directed mutagenesis of a cloned gene
Introduce or revert mutations or delete single to multiple bases
Upto  5  mutations  within 10 bp stretch are counted as single service
Sequence confirmed clone delivered

SB/MUT 01

Random mutagenesis of a cloned gene
Introduce random mutations in whole gene/part of genes/ domain of interest 50 random clones delivered
One clone sequenced to confirm random mutations introduced.

SB/MUT 02

siRNA Design and Synthesis

Designed and synthesized for different systems (Size: ~250-500 bp dsRNA, 3 Nos. per dsRNA, 50 µg)
siRNA and shRNA are synthesized
Site-less cloning performed for perfect transcript production, no extra bases

SB/SIR 01

SB/SHR 01

Drosophila siRNA

Available  for   silencing  all Drosophila genes individually
Validated to be gene-specific
Provided 10  µg enough for 10 or more experiments

SB/DSR 01