Ready-to- Ligate Cloning Vectors

Single or double restricted as per requirement                                                                                                                    
Calf Intestinal Phosphatase treated
Checked for no / low self-ligation

SB/PCN 34

20 Ligations

PCR Cloning Kit (T/A)

Cloning of PCR products, 10 reactions
Unpurified PCR products preferred for 
Cloning
Cloning efficiency varies for amplicons
Guaranteed clones with E. coli CB5a Competent cells 

SB/PCN 23

10 Reactions

Ligation Kit: Quick protocol

5 mins Ligation
Positive Controls (ready-to-ligate Vector & Insert) provided
Works best with E. coli CB5a Competent cells 

SB/RKN 63

SB/RKN 64

50 Ligations

100 Ligations

Competent Cells
E. coli (CB5 a)

Transformation efficiency: 5x106
Competent cells are send ready-to-use, can be stored at -70ºC for
2 weeks only, can not be stored at -20ºC
Please use 5-10 µl of ligation mix for best efficiency
The Competent cells are shipped on Dry-Ice

SB/PCN 30

SB/PCN 31

20 X 50 μl

10 X 50 µl

Cloning Kit (T/A) with Competent cells

T/A Cloning kit for 10 cloning reactions
10 vials of 50 µl competent cells
Competent cells are sent ready-to-use, can be stored at -70ºC for 2 weeks only, can not be stored at -20ºC
Shipped on Dry-Ice.

SB/PCN 32

10 Clonings

cDNA First-Strand 
Synthesis Kit

Starts from purified total RNA or mRNA
Oligo-dT or Random hexamer based synthesis using M-MuLV 
Robust RNAseH negative M-MuLV RT provided

SB/RKN 65

20 Reactions

cDNA Library Kit

Starts from purified total RNA or mRNA
Oligo-dT or Random hexamer based synthesis using M-MuLV 
Reverse RNase H negative M-MuLV RT provided
Robust RNase H negative M-MuLV RT provided
Adapter ligation
PCR based library amplification / Normalization
Cloning of amplicons/ Library in “Ready-to-ligate” vectors

SB/RKN 66

10 Reactions

DNA Labeling Kit: Random Priming

Random hexamer based
Can be adapted for radio-active or non- radioactive protocols
All Enzyme and reagents provided for 25 reactions

SB/RKN 67

25 Reactions

DNA Labeling Kit: Nick Translation

DNase-1 created Nicks polymerized using DNA polymerase 1
Can be adapted for radio-active or non- radioactive protocols
All Enzyme and reagents provided for 25 reactions

SB/RKN 68

25 Reactions

Competent Cell preparation kit

All Reagents for preparing 50 vials of 100 µl (5 ml) competent cells
Transformation efficiency of 5 x 106 efficiency achievable
Competent cells, once made, can be stored at -70ºC for 2 weeks only, can not be stored at -20ºC
Please use 5-10 µl of ligation mix for best efficiency

SB/PCN 33

50 X100 μl

Silver Staining Kit: DNA 

Detects ~10 ng of DNA
25 min. protocol
High sensitivity
Kits for 10 and 25 gel staining

SB/RKN 69

SB/RKN 70

10 Gels

25 Gels