Shrirent Botech - an integrated Molecular Biology / Biotech Services company serving R & D and academic Community and Scientists.

          PCR Enzymes and Kits: FastPCRTM

          FastPCRTM Reagents: Perform routine and complex PCRs in flat 40-50 min.
          Get 4 to 5 FastPCRTM runs in the time of one Standard PCR run
          Fast Q-PCR Reagents: Perform all routine and complex Q-PCRs in Fast and Standard formats

Product	Specifications	Catalogue No	Pack Size
FasTaq™ Polymerase	Polymerizes @ 500 bp/ sec Just 1 sec extension for 500 bp amplicon Just 2 sec extension for 1 kb amplico FastPCRTM cycle condition: 500 bp FastPCRTM cycle condition: 1 kb Fidelity similar to Taq Polymerase The enzyme works in all standard PCR Machines Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc. PCR Controls (template and primers) provided	SB/FCN 01	300 Units
FasTaq™ Polymerase
FasTaqHiFi™ Polymerase	Fidelity similar to Pfu Polymerase Polymerizes @ 500 bp/ sec Just 1 sec extension for 500 bp amplicon Just 2 sec extension for 1 kb amplicon FastPCRTM cycle condition: 500 bp FastPCRTM cycle condition: 1 kb The enzyme works in all standard PCR Machines Good for all PCR applications: Routine PCRs, Nested PCR, Multiplex PCR, etc. PCR Controls (template and primers) provided.	SB/FCN 02	300 Units
FastLong™ Polymerase	FasTaq and FasTaqHiFi Enzyme mix for better fidelity High Fidelity: 13x10-6 Polymerizes @ 500 bp per sec. Just 30 sec extension for 5 kb amplicon FastPCRTM cycle condition: 5 kb The enzyme works in all standard PCR Machines Amplifies upto 5 kb PCR Controls provided	SB/FCN 03	300 Units
FastPCR™ Master -Mix	FasTaqTM Polymerase –based kit FastPCRTM Cycle conditions: 40 min for 30 cycle PCR All PCR components added at 2X concentration, just add template, primer and water to start FastPCRTM	SB/FCN 04	5 x 1 ml

Standard PCR

Product	Specifications	Catalogue No	Pack Size
Taq DNA Polymerase	3U/ µl, buffer with MgCl2, PCR Controls provided	SB/PCN 01	300 Units
	3U/ µl, buffer with MgCl2, PCR Controls provided	SB/PCN 02	2 x 300 Units
	3U/ µl, buffer with MgCl2, PCR Controls provided	SB/PCN 03	5x 300 Units
	1U/ µl, buffer with MgCl2, PCR Controls provided	SB/PCN 07	2 x 300 Units
	1U/ µl, MgCl2 provided separately, PCR Controls provided	SB/PCN 08	2 x 300 Units
	1U/ µl, MgCl2 provided separately, PCR Controls provided	SB/PCN 09	5 x 300 Units
	3U/ µl, MgCl2 provided separately, PCR Controls provided	SB/PCN 04	300 Units
	3U/ µl, MgCl2 provided separately, PCR Controls provided	SB/PCN 05	2 x 300 Units
	3U/ µl, MgCl2 provided separately, PCR Controls provided	SB/PCN 06	5x 300 Units
GreenTaq™ Polymerase	Green colored Taq for easy viewing and loading 3U/ µl, Buffer contains MgCl2 PCR positive controls provided with every purchase	SB/PCN 13	300 Units
GreenTaq™ Polymerase	Green colored Taq for easy viewing and loading 3U/ µl, MgCl2 provided seperatly PCR Controls provided with every purchase	SB/PCN 14	300 Units
Fidelity Polymerase	Highest known Fidelity: 1.5 x 10-6 (1.5 mutations in a million bases incorporated Similar to Pfu Polymerase PCR positive Controls provided with every purchase	SB/PCN 10	300 Units
ChromTaq™ Polymerase	Enzyme mix of Taq polymerase and Fidelity polymerase High Fidelity: 13 x 10-6 Can be used for all regular PCRs Amplifies up to 5 kb PCR positive Controls provided with every purchase	SB/PCN 11	300 Units
XTRA™ Polymerase	For 3-20 kb amplification For usage with AutoExcel PCR Protocol PCR positive controls provided with every purchase	SB/PCN 12	300 Units
PCR Master- Mix (2 X)	Taq Polymerase-based kit 2X-mix (make up volume with template, primers and water)	SB/PCN 24	5 x 1 ml
ChromTaq Master-Mix	ChromTaq-based high fidelity PCR kit, provided as 2X 2X-mix (make up volume with template, primers and water)	SB/PCN 17	5 x 1 ml
GC-PCR Solution	Good for GC-rich repeat amplification (Fragile-X) Good for amplification of GC-rich genes (genes from M. tuberculosisgenome)	SB/PCN 18	1 ml
Long-PCR Master-Mix	5-20 kb amplification kit for plasmids/ genomes	SB/PCN 20	5 x 1 ml
GreenTaq Master Mix	GreenTaq™ Polymerase-based kit 2X-mix (make up to volume with template, primer and water)	SB/PCN 19	5 x 1 ml
RedTaq polymerase	Red colored Taq for easy viewing and loading 3U/ µl, Buffer contains MgCl2 PCR positive controls provided with every purchase Red colored Taq for easy viewing and loading	SB/PCN 15	300 Units
RedTaq polymerase	Red colored Taq for easy viewing and loading 3U/ µl, MgCl2 provided separately PCR positive controls provided with every purchase Red colored Taq for easy viewing and loading	SB/PCN 16	300 Units
RedTaq Master-Mix	Red Taq Polymerase -based kit 2X -mix (make up volume with template, primers and water)	SB/PCN 25	5 x 1 ml
AMV Reverse Transcriptase	RNA directed DNA polymerase, synthesizes first strand cDNA Can reverse transcribe long RNAs (up to 10 kb) Success of reaction heavily depends on RNA quality	SB/MFE 09	100 Units
M-MuLV Reverse Transcriptase	RNA directed DNA polymerase Native enzyme, has RNaseH activity Synthesizes first strand cDNA Can reverse transcribe RNAs upto 3 kb long Success of reaction heavily depends on RNA quality	SB/MFE 10	1000 Units
M-MuLV Reverse Transcriptase (RNase H Negative)	RNA directed DNA polymerase Genetically modified, RNase H negative Synthesizes first strand cDNA Can reverse transcribe RNAs up to 7 kb long Success of reaction heavily depends on RNA quality	SB/MFE 10 A	1000 Units
RT-PCR Kit (M-MuLVbased)	For mRNA/ cDNA of ~3.0 kb Robust amplification 5 control reactions	SB/PCN 28	10 Reactions
RT-PCR Kit (M-MuLV, Single tube)	For mRNA/ cDNA of ~3.0 kb RT and PCR are performed in same tube Robust amplification 3 control reactions	SB/PCN 29	10 Reactions
RT-PCR Kit (AMV)	For mRNA / cDNA of >3.0 kb Robust amplification 5 control reactions	SB/PCN 26	10 Reactions
RT-PCR Kit (AMV, Single tube)	For mRNA/ cDNA of >3.0 kb RT and PCR in same tube Robust amplification 3 control reactions	SB/PCN 27	10 Reactions
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